Volume 12, Issue 4, Autumn 2012, Page 1-173


Histopathologic and Histochemic study of mice infected with Giardia

Alkennany; E. R. Al

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 17-31

This research was conducted to investigate the histopathologic and histochemic changes of small intestine and hepatic tissues of mice infected with Giardia lamblia. Results illustrated variable changes of intestinel represented by blunting and shortening of villous in the mucosa associated with hyperplasia and hypertrophy of epithelium in addition to localization of different stages of parasite. In hepatic tissue lesions characterized by diffuse vacuolar degeneration in hepatocyte and bile duct epithelium, also apoptosis has been seen concomitant with presence of parasite. Histochemically infected intestine showing a strong positive reaction with both PAS and AB (pH2.5) stains denoting an increase in mucopolysaccherid . In heptocyte strong positive reaction with both PAS and best cermine Also observed.
The present study suggest that infection of mice with Giardia is (firstly time reported) communicated with alteration in tissue due to biochemical changes that produced from the interaction between parasite and cellular components.

Antimicrobial activity of Lactobacillus spp. metabolites on growth of two clinical isolates in vitro

Eiman A. Saeed

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 1-8

Antimicrobial activity of secondary metabolites that produced by Lactobacillus spp. has been investigated against two clinical isolates of Escherichia coli and Pseudomonas aeruginosa.
Acidity effect of anaerobic fermentation of lactose in raw milk to lactic acid by Lactobacillus spp. was studied. The accumulation of acid in the medium lead to lowering pH of the yoghurt that prevent growth of E. coli and P. aeruginosa. The ability of two isolates was experimented for survival in acidic, alkaline, and neutral pH, the results showed that E. coli and P. aeruginosa have no growth in acidic pH while they have heavy growth in neutral and alkaline pH.
This study confirmed the presence of synergistic effect between the secondary metabolites of lactobacilli and the acidity in yoghurt, which lead to inhibitory effect on the growth of two clinical isolates.
Key ward: Lactobacillus, probiotics, secondary metabolites, therapeutic effect

Extraction of nikel (II) frome aqueous solution by using 2-[α- Naphthyl azo ] -4,5-diphenyl imidazol

Ghusoon .F. Hameed

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 92-103

Extraction of nikel (II) from aqueous solution by use new ligand 2-[α- Naphthyl azo ] -4,5-diphenyl imidazol (α-NADPI) which is prepared as in study [1] is proposed as a sensitive and selective analytical reagent for the extractive spectrophotometric determination and extraction of Ni+2, this study shows the optimum pH for extraction Ni+2 was (pHex= 9) , also the two layers need(10 minutes) for shaking to reach the novel equilibrium ,as well as the optimum concentration of Ni(II) for high extraction ability was ( 30 μg Ni+2 ) (1.022 x 10-4 M) ,stoichiometric study shows the more probable structure of ion pair complex extracted was(1:1) (metal: ligand ) [Ni (α-NADPI)]+2 (Cl-)2 , so that organic solvent studies shows there is not any linear relation between distribution ratio (D) and dielectric constant (Ɛ) for organic solvents used,at later thermodynamic study shows the reaction between Ni+2 ions and ligand( α-NADPI) was endothermic reaction

Nosocomial infection caused by Methicillin- Resistant Staphylococcus aureus from different positions of Al-Fehaa Hospital-Basrah

Mehammed A.I.Almazin; Kuther H.Mehdi

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 32-38

Ninety bacteria isolates were obtained from different areas in Al-Fehaa Hospital, this represents the operative theaters, wards and hallways of the hospital, the present studies showed that the prevalence of 27 Methecillin-resistant Staphylococcus aureus (MRSA) in nosocomial infections and their antibiotic resistant pattern to (Calvulanic acid, Amoxicillin, Clarithromycin, Vancomycin, Cefotaxin, Gentamycin)in concentrations (15, 20, 10, 30, 10, 30, 30)µg/ml at respectivily.
The isolates of MRSA were identified by biochemical tests and all isolates of MRSA were resistant to Vancomycin (30µg/ml), Amoxicillin (30 µg/ml), Gentamycin (10µg/ml) and Clarithromycin(10µg/ml) .

Preparation of copolymer of acrylamide and acrylic acid and its application for slow release sodium nitrate fertilizer

Rafid K. Kmal; Prof.Ass.Dr.Mohammed A.Mutar

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 71-83

In this search The hydrogels were prepared by free-radical crosslinkig copolymerization of the monomer acrylamide (AAm) with acrylic acid (AA) as a crosslinker in aqueous solutions in the presence of potassium persulfate (KPS) as initiator and N,N,N',N'-tetramethylethylene-diamine (TEMED) as accelerator, polyacrylamide hydrogels structures were prepared by using acrylic acid (AA) (0.5, 1, 1.5, 2, 2.5) gm in the presence N,N'-methylene-bisacrylamide (MBA) as crosslinking agents at temperature 60 0c for 2 hours.
The swelling ratio (Rs) was measured for all the hydrogel structures in aqueous solution as function of time at room temperature. Sodium nitrate was loaded into polymeric matrix during in situ polymerization. The concentration of sodium nitrate released was measured on flame photometer every 1 hr. The fertilizer release studies from the sodium nitrate loaded hydrogel was studied in aqueous solution as function of time at room temperature. The results showed that the samples involving the highest release of sodium nitrate were arranged as follows:
PAAm-AA2.5gm > PAAm-AA2gm > PAAm-AA1.5gm > PAAm- AA1gm > PAAm-AA0.5gm

Adsorption of Nickel Ions From Aqueous Solutions by Green Tea Waste

Mohanad Hazim Naji HALBOOS

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 59-70

In this research, the green tea waste (GTW) was utilized for the removal of Ni(II) ion from aqueous solutions. Adsorption experiments were conducted under various conditions where the batch method was used to determine the best adsorption ratio and the adsorption capacity for the concentration used of Ni(II) ion between 10-100 mg/L, the results show that the best concentration was 10 mg/L where the adsorption ratio was 92% and the adsorption capacity was 2.3 mg/g. The adsorbent dose was 4 g/L, and the shaking time found that it equal to 60 min and the best pH value between (3-8). The effect of temperature and thermodynamic functions wear also studied, the adsorption capacity was increased with increased the temperature and the reaction was endothermic. The equilibrium isotherm data was analyzed using the Langmuir, Freundlich and Tempkin equations. The rate constant k2 for the adsorption of Ni(II) ion is equal to 0.0571 g/mg.min.

(Study of the Influence of Cigarette Smoking on Serum Oxidant and Antioxidant Status)

Safaa H. A. Al-Ghzi

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 130-141

The use of tobacco is a word wide problem and the traditional method of tobacco use is smoking cigarettes. This study was aimed to investigate the balance state in oxidation system (by measuring malondialdehyde (MDA) levels lipid peroxdation marker) - antioxidant system (by measuring ceruloplasmin (Cp) antioxidant marker) and attempted to shed a light on the possible correlation between (MDA), (Cp) and age of smokers and period of smoking.
Sample size was (49) healthy adult men smokers between the ages of 19-32. The results showed a presence of a significant increase in MDA levels in all smoker groups (P ≤ 0.05) in comparison with control group (nonsmokers). Also, the results evinced the presence of a significant decrease in ceruloplasmin levels (P ≤ 0.05) in comparison with control group (nonsmokers).
Our results confirmed the role of reactive oxygen species (ROS) in development of oxidative stress disorders, where serum MDA was found to be positively correlated with period of smoking. Whereas the results showed the presence a negative correlation between ceruloplasmin and period of smoking. Also proved no relationship between age of smokers and MDA levels and Cp levels.

Keywords: Somking, Oxidants, Antioxidants, Malondialdehyde, Ceruloplasmin.
* Assistant lecturer, Biochemistry, Al-Shatra technical institute

TWO FIXED POINT THEOREMS IN ORBITALLY COMPLETE GENERALIZED METRIC SPACE

Dr. Salwa Salman Abd

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 142-155

The aim of this paper is to prove two results about the existence of unique fixed point for self mappings defined on an orbitally complete, (or, on an orbitally complete chainable) generalized metric space.These results based up on general contraction mapping and locally general contraction mapping which include some known results as corollaries. Therefore, our results unify and extend the results in Das[1], Das and Day[2] and C’iri’c[4].
Key words: generalized metric space, fixed point, orbitally complete, locally contractive mapping, є -chainabl

Synthesis, Characterization, and Antibacterial effect of Complexes lead(II) and Tin (II) With (L-leucin and 8-hydroxyquinoline)

Taghreed H. Al- Noor

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 104-112

The research includes the synthesis and identification of two types complexes of M+2 Ions using amino acid( L-leucin) and 8-hydroxyqinoline as ligands in general compositions [ M (Leu)2] and
[ M (Q)2] were L-leucin (C6H13NO2)symbolized as (LeuH), and 8-hydroxyqinoline,(C9H7NO2) symbolized as (8-HQ), M(II)= Sn(II) and Pb(II), The ligand and metal ions were brought into reaction in ethanolic medium using (1:2) metal : ligand molar ratios as required.
Products were found to be solid crystalline complexes, which have been Characterized through the following techniques: Melting points, Solubility, Molar conductivity. Determination the percentage of the metal in the complexes by (Atomic Absorption Spectrometry). chloride ion content were also evolution by (mohr method) . Spectroscopic Methods [FT-IR and UV-Vis]. The proposed structure of the complexes using program , chem office 3D(2006) .

Role of vitamin C in decrease the toxic in lead acetate and some physiological and Biochemical parameter in male rats.

Dr. Ibrahim O.S. AL-Quraishi

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 63-75

The present study aimed to the role vitamin C in of reducing the toxic effects result from exposing to lead acetate. Male rat (Albino Rats) used the study, Injections interaperitoneal the lead acetate of concentration (4, 8, 12 mg/kg) for 4 weeks. Also administration vitamin C concentration (14 mg/kg) in the synchronically an non-synchronically manner of the exposure to lead acetate of concentration (4, 8, 12 mg/kg).
The result studied according to the following:
1- Significant decrease (P<0.05) of RBC count, Hb concentration and PCV. especially in high concentrations of the experiment.
2- Significant increase (P<0.05) of total WBC count, Neutrophils and eosinophil count, especially in high concentration of the experiment. Beside significant decrease (P<0.05) of Lymphocytes count, but Monocytes increment the significant level (P>0.05) .
3- Significant decrease (P<0.05) of serum total protein, concentration which is increased by increase of concentration of exposure, significant increase (P<0.05) of cholesterol concentration, Triglyceride, and VLDL-C in serum of the high concentration of lead, while HDL-C its concentration significant increased (P<0.05) .
The receiving vitamin C in a synchronically with ascending concentration lead acetate led to efficient improving in parameters including study in group (G3, G6, G9) the toxic effects of lead acetate and relieving is toxicological changes comparing with groups (G1, G4, G7)which were injected by lead only, or group administration vitamin C non synchronically (G2, G5, G8) the some the samples taken from the animals of the control group.The administration vitamin C in a non synchronically with lead acetate not led to any improving clearing in studying parameters especially in groups (G5, G7).Conclusions of study to of exposing to different concentration of lead acetate synchronically because effects negative in the physiological . improve this study administration vitamin C synchronically to role relation effect lead acetate

Effect of some environmental factors on the tolerance of Bacillus subtilis to heavy metals

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 38-48

Twelve isolates of bacteria were obtained from samples of different soils and water amended with 100μg/ml of five heavy metals chlorides (i.e: Aluminum Al+2, Iron Fe+2, Lead Pb+2, Mercury Hg+2 and Zinc Zn+2). Four isolates were identified as Bacillus subtilis and B. subtilis (B2) isolate was selected for this study according to their resistance to all five heavy metals chlorides. The ability of B. subtilis (B2) isolate for growing in different concentration of heavy metals chlorides ranging from 200-1200 μg/ml was tested. The highest conc. that B. subtilis (B2) isolate tolerate was 1000 μg/ml for Al+2, Fe+2, Pb+2, and Zn+2and 300 μg/ml for Hg+2 for 24hour.The effect of heavy metals chlorides on bacterial growth for 72 hrs was studied. B. subtilis (B2) isolate had not affected by presence of pbCl2 FeCl2and AlCl2 in growth media. However, it was slightly affected by ZnCl2 during incubation period while mercury causes no bacterial growth. The effect of temperature on bacterial growth in media with heavy metals was studied. B. subtilis (B2) isolate showed growth in temp. range (30 - 50) ºC with the present of the four heavy metals chlorides Al+2, Fe+2, Pb+2and Zn+2 while there are no growth in the presence of Hg+2 at all temperatures. The effect of different pH values on growth of B. subtilis (B2) isolate in media with heavy metals was studied.
Results showed the ability of B2 isolate for growing in different pH values 4,7,9 with presence of the four heavy metals chlorides Al+2, Fe+2, Pb+2and Zn+2 in all pH values at conc. 1000 μg/ml and unability for growing in media with 300 μg/ml Hg+2. The ability of B. subtilis (B2) isolate for removal of heavy metals chlorides was studied and showed that the highest Zn+2 removal ratios 75% then Fe+2removal ratio 71% while Pb+2 has the lowest removal ratio 37%.

Preparation of UO22+ as ion selective electrode depend on active substance dibenzo-18-crown-6 (DB18C6) by using PVC polymer

Abbas noor Al-Sharify

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 116-128

A Uranyl (II) ion- selective electrode was prepared, depend on the active material Dibenzo-18-crown-6 ( DB18C6) as a sensor, deposed on a poly vinyl chloride ( PVC) polymer, by using Di-n-Octyl phenyl phthalate (DOPP) as a ,plasticizer substance in the membrane, the behavior and characteristics properties of this electrode have been studied.
It respond for the concentration range (1×10-7-1×10-1) mol/L of uranyl ion with nernast linearity slope equal to 29 mV/decade of activity and correlation coefficient was about (0.9990).
The selectivity coefficient K_(i,j)^potof this electrode was calculated, in the presence of some interferencing cations and anions with certain uranyl (II) ion solutions.
It was found that the range of pH response is (4-9), with response time (25) sec. at (298) K, and the electrode lifetime was found (28) days.
Key words: Ion Selective Electrode, UO22+ , DB18C6 .

A Study of Helicobacter pylori pathogenesis in laboratory mouse

Dr.Maitham Ghaly Yousif

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 88-103

Helicobacter pylori is considered one of the most etiology of active chronic gastritis, peptic ulcer, ulcer and inflammation of the duodenum, gastric cancer, and tumors associated with the mucous layer.
Gastric biopsies were collected from(80)a condition subject to examination endoscoby (42 males and 38 females) with differenced ages, in Deywanyia Teaching Hospital for the period 25/10/2009 until 09/03/2010 diagnosed 77 (96%) condition. The biopsies were taken from each patient from the mucous layer of the gastric antrum to investigate the bacteria H. pylori used one to check urease rapid and the other to isolate the bacteria into the center of DENT′S selective medium, which is the best center in the first isolation compared to other media.
The study showed the efficiency of test urease rapid screening for bacteria, as it was able to prove the presence of bacteria in 43 (54%) biopsy tissue, while the isolation of bacteria from 18 (23%) condition mediated transplant bacterial and under conditions of low ventilation, isolates genetically has been typing using specialized primers to the detect gene that represents the gene UreaC and equal (820) base pairs was spectrum using technology which is the most quality and sensitivity is a technology interaction polymerase serial (PCR) Polymerase Chain Reaction, where the local strains which secrete urease were grown, then genetic material isolated and purified DNA was from it to use in the investigation of gene UreaC responsible for the secretion of urease. The identification was made by direct bacterial H. pylori from the saliva of patients where it was purification and extraction of genetic material the DNA of the bacteria without the development and use of primers, specialized gene target Urea C and after amplification of this gene identified in four cases only satisfactory.
The Histopathological studies of H. pylori in laboratory animals (mice) BALb / C mice showed the emergence of changes in tissue degenerative severe included the appearance of infiltration of cells, inflammatory, including cells multiple nuclei (Polymorph nuclear cells) (PMNs), (Lymphocyte cells) and (Plasma cells) in the lamina propria layer mucosa and the gastric glands, as demonstrated by degeneration of water in the epithelial cells of the layer of mucus and gastric glands to the point of increasing the volume of glands Hypertrophy with a erosion in the surface layer of the part of the mucosa and a case of congestion of the blood vessels with a case of vaculation in a number of epithelial cells infectious diseases, as well as the third group show the state of cellular degeneration and the onset of ulceration in the stomach mucosa, compared with the control group that did not show any tissue change.

The effect of Diablo (imidaclopride) on the survival of Branchiura sowerbyi Beddard, 1892 (Annelida: Oligochaeta)

Najem Abdul Wahid Aljaduu

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 14-20

This study aimed at the effect of Diablo pesticide on the survival of aquatic oligochaets Branchiura sowerbyi in the laboratory using three concentrations of the pesticide and they are (0.5, 0.25,0.125)% and doubled the recommended and half, respectively, and have shown that the results of the pesticide and toxic effect of a killer the worms as the cause of loss of all the worms through (1,1.5,1.6) minutes of exposure to concentrations prepared and respectively comparing with the controlled group ,thus that the pesticide impact on the behavior of the worms through the irritation and springing around itself. Moreover, the results of statistical analysis have shown a significant inverse relationship between the concentrations pesticide used and the time loss worms, and the value of LC50 ranged between (0.000002-0.000003)% m/l.

Study of ovarian tumors in patients sample in Missan Governorate

Nidhal Abdullah Hashim

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 28-35

This present study was performed on (40) patient of ovarian tumors in age (20-60)year to investigate the age group which more expose for ovarian tumors accident and the relation between age and tumor nature(benign or malignant). in addition to known the symptoms of disease and detection the types of benign or malignant ovarian tumors, that during period 1/11/2010 to 1/4/2011.
This study show increase percent of ovarian tumors in age group (20-30)year in percentage 35% follow by age group (31- 40)year in percentage 32.5% while was age group (51-60)year lower percent 12.5%, important symptoms of disease was the accut pain in abdomen in percent 62,5% follow by abdomen swelling in percent 17.5% and gastric and intestinal disorders in percent 12.5% in addition to menstruation disorder and urinary infection but in lower percentage .
Record the benign ovarian tumors 80% compared with malignant ovarian tumors 20 % in addition to correlation between age and tumor nature ,the age group (20-30)year record higher percent in benign ovarian tumors 43.7% follow by age group (31-40)year in percent 37.5% while age group (51-60)year record lower percent 3.1% in benign ovarian tumors while it record higher percent 50% in malignant ovarian tumors follow by age group (41-50)year in percent 37.5%.
This study show the benign ovarian tumors types ,record benign serous cyst adenoma percent 40.6% follow by poly cystic tumor in percent 28.9% while lower percentage was luteal cyst tumor 6.25% while malignant ovarian tumors record Serous cyst carcinoma tumor 50% follow by Immature(malignant) teratoma tumor in percent 25% while type Clear cell carcinoma,cyct teratoma tumor record lower percent 12.5% in one patient for each .

isolation of Toxoplasma gondii histocysts from Local Chickens , Wild and Domestic Pigeons

Hadi M. Hamza

journal of al-qadisiyah for pure science(quarterly), Volume 12, Issue 4, Pages 36-47

The present study conducted to isolate and diagnose Toxoplasma gondii in three species of birds include Gallus gallus domisticus (150 samples), Columba livia domisticus (130 samples) and Columba livia(130 samples) to be the total number 410 samples during the period from October 2010 to May 2011. The samples was collected from different regions of the Al-Diwaniya province. the study aimed as well as to isolate and diagnose Toxoplasma gondii, study infection rates and severity and the role of birds in the transfer toxoplasmosis to human.
The results of laboratory tests based on the latex agglutination test (LAT) showed presence of toxoplasmosis infection disease caused by parasitic Toxoplasma gondii with different rates. The results of immunological tests for 150 serum samples from Gallus gallus domisticus showed that 49 samples were positive with rate 32.6%. While the results of examination of 130 serum samples from Columba livia domisticus showed that 32 samples were positive with percentage 24.6%. while the results of examination of 130 samples serum from Columba livia showed that 42 samples were positive with percentage 32.3%.
The tissue cysts have been isolated from number of organs (heart, brain, lungs and liver) of the birds studied were positive for the latex agglutination test (LAT) by using dissecting. The results showed that the highest rate of infection was in the liver (34.6%) in the Gallus gallus domisticus and 28% in the Columba livia domisticus and 19% in the Columba livia. As well as the egg cyst was isolated from the intestines of a bird infected during the autopsy. The results of the current study also revealed a major role of domestic birds in transfer of toxoplasmosis infection to humans as one of the common diseases among humans, animals through determination the parasite specific gene in the serum and isolation of the tissue cysts from the organs.
The current study is considered the first in Iraq about the Toxoplasma gondii in these birds as well as isolation of tissue cysts from its organs is also the first kind in Iraq.