Volume 16, Issue 1, Winter 2016, Page 1-201

Study the effect of thermal change in some biochemical parameters in male rats infected with diabetes mellitus experimentally

Ihsan Raisan Ibrahim

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 10-23

The current study aimed to knowing the effect of different temperatures degrees for different periods on some biochemical parameters on diabetes rats. Two experiments were carried out, the first is hyperthermia 80 rats (40 healthy and 40 infected) were divided into five groups( each group subdivided into: 8 health and 8 infected). First group considered as a control group exposed to normal temperature (21 ± 2) c °, the second, third , fourth group exposed to high temperature (40 ± 2) ° C and divided two groups infected and healthy for one hour, the third group: offered to degree (40 ± 2) ° C for one, tow, four hours respectively. Secondary experiment is hypothermal it was designed in same design of first experiment and temperature used was (5 ± 2) ° CThe results showed significant increase (P <0.05) in the level of glucose, cholesterol , triglycerides ,lipoproteins and GOT in healthy group and infected and in GPT in healthy group exposed to high temperature. All group showed significant increase in all infected parameter (P <0.05) compared whit healthy group in one hour period. Unless HDL which suffered from decrease.
. As at increased exposure to two hours, he got the same results in the previous period except significant increase (P <0.05), which happened in the level triglycerides and VLDL in the group exposed to low temperature and decrease, which happened in LDL a infected group exposed to low temperature compared with healthy group
In the period of four hours the results were similar that in the period one hour except the significant decrease (P <0.05) in cholesterol and HDL and GOT in the group exposed to low temperature compared with healthy group
Levels of glucose and ALP and GOT increased in healthy animals and infected in groups expose low temperature, also HDI In LDLV significantly increase in group exposed to high temperatures, there was significantly differences in levels of cholesterol and triglycerides and HDL in the group exposed to high temperature also LDLV in group exposed to low temperature. Depending on period of of exposure

Study of Effect of Sodium fluoride on Male Reproductive system and some blood Parameters in Albino Rabbit

Hashem M. A-Kareem

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 1-9

The present study has been carried out to Know the toxic effect of sodium fluoride on Albino rabbit males , for purpose study the histological Changes which include (Testis),moreover study the changes in some blood parameters and biochemistry and testosterone level . 10 Albino adult male rabbits were used in this experiment aging about (10-14) months the animals were treated with oral swallow for 12 weeks . The animals were divided to four groups : control group is oral treated with (0.9%) normal saline. First group was treated with 10 mg/kg of body weight sodium fluoride , second group was treated with 20 mg/kg of body weight sodium fluoride and third group was treated with 30 mg/kg of body weight sodium fluoride .
The results of present study showed that the significant decreased in testosterone hormone level in the treated animals with sodium fluoride compared with control group ,while the histological results showed that the histological changes and reduction or freezed stages in spermatogenesis processes which timed with decreased in number of primary spermatocytes and secondary spermatocytes in addition to present of lymphocytes , also showed decreased in epididymus ducts and decreased number of sterocilia and decreased number of mature sperms.cholesterol, urea, transporter enzymes for (GOT, GPT) and bilirubin.It has been concluded that the animals treated with sodium fluoride lead to histological changes of organs and induction changes of some blood parameters and biochemical specially that all severe changes increased with increased concentration and time of exposure .

Immunohistochemical study for localization detection of insulin like growth factors(IGF1) in testiscular tissue of immature and mature male Wistar rats

jJabbar A. A. Al-Sa; aidi

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 24-31

The present study has been carried to investigate the immunological localization of Insulin-like Growth Factor 1 (IGF1) in pre- and post-pubertal stages and its involvement in male reproductive physiology of rats,
Forty immature and mature male Wistar albino rats were used in the present study which assigned into 5 equal groups, 8 per each at 25th, 35th, 45th, 55th, and 65th day of age, male rats of each group have been anaesthetized. Testes tissue samples were obtained for immunohistochemical study.
The results of immunohistochemical study showed the presence of IGF1 immunoreactivity in,sertoli cells, leydig cells and all cells that Constituent of seminiferous tubule, and showed density immunoreactivity increase during pubertal stage. Also observed the presence of IGF-1 immunoreactivity in mature sperm in seminiferous tubule lumen of post-pubertal testis.

Bioremediation for Ochratoxin A by Using Some Species of Bacteria isolated from Iraqi Dates

Basaid A.Zaid

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 32-49

This study aimed to evaluate the effectiveness of some species of bacterial isolated from Iraqi Dates in the treatment of toxic Ochratoxin A and the possibility of appointment one isolate them as us drug country poison Ochratoxin A In Invivo.
The study results showed the effectiveness of the bacteria Lactococcus plantrum in reducing the toxicity of Ochratoxin A externally (Invitro) since disappeared shine spot poison Ochratoxin A treatment with bacteria from the plate Thin layer chromatography when exposed UV , reinforced this result vital tests that took place inside the body of animals albino Rat and the transaction of Ochratoxin A pre- treatment bacterium Lactococcus plantrum that were in organ of the studied included the kidneys, completely intact while showed pathogenic changes clear in those animals treated with the organs of Ochratoxin A untreated bacteria.
It also proved of the formula manufacturedfrom bacteria L. plantrum and debilitating heat highly effective in reducing the toxicity of Ochratoxin A demonstrated by the survival of all of the level of hemoglobin ,Keriatenin and urea the levels of these parameters were (14g/ml,23 g/dl and 65g/dl)respectively /, while the level s of above parameters in animals treated of Ochratoxin A only( 9g/ml,43g/dl and 320g/dl) respectively.
On the other hand it was the preparation of vital important role in defense the tissues of animals treated by followed by treating them Ochratoxin A, as results showed histological examination of sections of textile for kidneys safety of any pathological changes , in the time it appeared pathological changes severe in all those organs of the animals organs tissues treated by Ochratoxin A only was the emergence of hypertrophy of glomerulus, necrosis and cells death of the tubules renal in kidneys.

Antibiogram of Staphylococcus aureus from Reproductive Age Women

Rana S. Al-Taweel

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 50-57

The current study had included collection of 30 vaginal swabs from non pregnant reproductive age women in the Teaching Hospital of Maternity and Pediatrics in Al-Diwaniya city for the isolation of Staphylococcus aureus and studying its sensitivity toward seven antibiotics. Bacterial isolates had been identified according to culture features and microscopic characters in addition to the differential tests and the growth on selective media. A total of seven isolates of S. aureus had been isolated.
Discs diffusion method had been used for antibiotics sensitivity test. It was found that most bacterial isolates were multidrug resistant, where 100% of isolates were resistant to penicillin, oxacillin, tetracyclin, erythromycin, and clindamycin. Also 71.4% and 57.1% of isolates were resistant to gentamycin and vancomycin respectively.

Epidemiological Study of Cryptosporidium parvum in Al- Diwaniyah province

Reim Hameid Al-Shepany

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 58-70

The present study aimed to investigate the parasite Cryptosporidium parvum in the children under 12 years old in the province of Al- Diwaniyah and know the impact of some epidemiological factors in the spread of the parasite.The study includes the test of 1177 samples of feces from children for the period from. 2012.11.1 up to.2013. 10.31. Acid fast stain was used for the detection of Cryptosporidium oocystes.
The results of this study showed that the total infection rate of parasite reached ( 18.01% ) , with significant differences (P=0.003) during the months of infection with the highest rate of infection was recorded in the month of July (28.89%) and the lowest in the month of February(8.49%), Also recorded significant differences of infection for the age groups (P = 0.029) as it recorded the highest rate of infection among the age group (<1) amounting to( 21.52%) and lowest infection rate among the age group( 9-12) years as it was (10.79%) , also There is significant difference(P=0.281) betwee male and femal infection as the percentage of infection in the male (16.96%) while in the femal was ( 19.41%) , Also recorded significant differences according to the nature of the infection housing (P = 0.009) with the highest percentage of infection in rural (21.11%) and the lowest rate of infection was recorded in the city center (15.21%) , Also recorded significant differences for the presence of animals (p = 0.005) recorded the highest rate of infection in the people which have animals (cows) (21.64%) and the lowest rate of infection found in the people which do not have the animals (12.56%) , Also found that the parasite Entamoeba histolytica was the most parasite found with C. parvum.

Evaluate the use of certain industrial wastes supported molass Kosat for the development and production of oyster mushrooms Pleurotus ostreatus and its impact on some of the criteria for the production of winning

Samir Abdul Amir Saadoun

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 71-86

Study was carried out in order to determine the possibility of using waste plant starch / AL-hashmyia of b (corn husks and fetus corn and AL-glutin or dry dirt) and remnants of Sugar Factory Maysan represented by (Baggasse and molasses) and remnants of plants molasses represented by (b Pomace dates) media of agricultural mushroom oyster Pleurotus ostreatus , as the material molasses and different concentrations (2 ، 4، 6 )% was added to the circles agricultural Assistant diet of oyster mushroom because it contains many types of sugars needed to grow mushrooms in, showed the results of the evaluation process circles agricultural after harvest moral superiority of some circles in the production of agricultural fruiting bodies and reflected on the criteria studied in the experiment and the extent of vulnerability in rural Zorai and concentrations of molasses, as it recorded the results of calculation efficiency vividness produce the largest number of fruiting bodies on the corn husks and Baggasse dates in all concentrations of a substance molasses as she was the highest rate of efficiency vividness in concentration of 6% (76.18 , 73.51)%, respectively Compared with wheat straw 56.24%. But when calculating the sum total on the basis of wet weight was observed superiority of the treatment of corn husks and Baggasse dates on the rest of other transactions and all concentrations compared with the straw of wheat, and when calculating the average weights of fruiting bodies of the fungus shellfish observed convergence results for all transactions and for all concentrations of molasses compared with the treatment of hay wheat. As for measuring the average lengths of the legs of fruiting bodies outperformed the treatment of corn husks in focus 6% 5.27 cm on all circles agricultural used in the experiment and compared with the treatment of hay wheat 5.50 cm, and reached the highest rate of dry matter of the mushroom oyster also, which outperformed the moral treatment of corn husks dates and Pomace (12.07, 12.11)%, respectively, compared with 12.16% wheat straw. It also showed the results of the production cycle of the relationship oyster mushroom by the media and agricultural concentrations molasses treatment outweigh the corn husks 86.20 days and 84.06 days Pomace dates Compared with the treatment of wheat straw 98.70 days.

Isolate and diagnose fungi contaminated seeds, nuts traded in local markets in the city off Al-Diwaniya

Saba A.K.Ziadi

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 87-95

This study amied to identify fungi that infect the seed nuts traded in local markets in the city of Diwaniyah and intended for human consumption , namely, ( walnuts, almonds, Cashew , Pistachio, and seeds of the mixed of nuts Castania ) as it has been to isolate and diagnose 8 kinds innate is (P.italicum, A.ocherous, A.niger, R.stolanifer , F.Solani, C.lunata T.harizanum), in addition to yeast (G. candida)on 5 circles agricultural is (Yeast extract agar, Malt extract agar, Potato Dextrose agar, Sabroud Dextrose agar, Simmon citrate agar) The results indicate the existence of significant differences in the proportion of presence fungi below probability of P <0.05, as the results showed the highest pollution fungi are the seeds of pistachios, followed by seed nut and was the highest frequency of the fungus P.italicum on the middle food PDA as the percentage of impressions ( 61.53 %) for seed nut ( 57.14 %) on the middle food (Yeast ea) the seeds of peanuts , but for the seeds of almonds log fungus R. stolanifer percentage frequency ( 100.00% ) on the middle (PDA) and mushrooms P. italicum percentage frequency ( % 72.72 ) on the middle (Malt ea) As for the seeds of the problem of the brand ( Castaa ) scored fungus T.harizanum the emergence ratio ( 100.00%) on AGRO circles ( SDA ) and (Malt ea) and record the appearance of G. candida yeast ( 100.00 % ) on the middle pea

*Identification of some Intestinal Helminthes In Barbu grypus, Liza abu and study of histopathological changes Result from infection

Asifa M.Y.Al-mahi

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 96-115

The present study has been conducted in four parts of Diwaniya (Diwaniya center, Shamiya,Hamza and Afaq). A sample of 945 fish of Barbus grypus and Liza abu has been examined . It was found that 205 of the Barbus grypus fish and 344 of the Liza abu were infected with parasitic intestinal worms. Some parasitic intestinal worms have been detected such as the Neoechinorynchus iraqensis in both fish types. The female paulisentus Bothriocephalus acheilognathi were found in the Barbus grypus and the larva of Contracaecum sp was seen in the Liza abu type In Diwaniya river had high infection of both fish types was 61.31% . The Barbus grypus fish were recorded with 70.11% while the Liza abu fish infection was 57.21%. The Liza abu infection in the four parts of Diwaniya as 56.30%
In September the infection percentage was higher by 63.21% in the two kinds of fish . while in February, December the infection rate was as low as 50%. It was also found that length categories of ( 26-24.1)cm of Barbus grypus had high percentage of 67.16% , infection with intestinal worms in thelengthcategory(>-30.1) was 22.22%.
As for the Lizaabuithada high perentage of infection in length category (12-10.1) cm , the percentage was 67.53%.The present study has discovered many pathological changes (visable and microscopic)caused by the intestinal worms , Among the visable changes was embolism and bleeding of the walls of the infected intestinal microscopic changes included an increase in the phlogistic and phagocytic cell and ruining of the internal tissue lining the intestines and atrophy villi.

Study therapeutic effect of sweet almond(Prunus amygdalus) suspension on serum glucose level and lipid profile of healthy and experimentally induced diabetic rats

Lubn A. Kafi

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 116-129

The current study aimed to determine some of positive effect of sweet almond suspension (SAS) in lowering blood glucose and lipid profile of experimentally diabetic male rats , by using aloxan .
Thirty adult male rats were randomly divided into eight equal groups as follows, nondiabetic non treated control group (NDNTC) ,diabetic non treated control group (DNTC), diabetic treated groups by using two dose of SAS (1.42 or 2.84 g/Kg B.W) (DT1 and DT2), treated control groups by using two doses mentioned above (TC1 and TC2),the results showed significant decrease in means values of animal body weight in DNTC,DT1,DT2, after one week of experiment , in comparison with NDNTC, while the DT1 and DT2 showed increase in body weight started from the 40 days of treatment compared with DNTC in the same period.
Also injection of aloxan in DNTC, DT1and DT2,caused significant increase about 2.5 times mean level of blood glucose after five days of the injection in comparison with NDNTC , after the same period , while treatment with SAS led to significant decrease in mean level of blood glucose after 20,40 and 60 days of treatment in comparison with DNTC in the same period .
The results showed that the dose 2.84g/Kg B.W. of SAS led to significant decrease in mean level of blood glucose than 1.42 g/Kg B.W. with 20,40 and 60 days in DT1 and DT2.
After five days of aloxan injection there was significant increase in Total Cholesterol (TC),Triglyceride(TG),Low Density Lipoprotein Cholesterol ( LDL-C)and Very Low Density Lipoprotein Cholesterol (VLDL-C) and significant decrease in High Density Lipoprotein Cholesterol (HDL-C) in DNTC ,DT1 and DT2 in comparison with NDNTC in the same period ,while the treatment with SAS in DT1 and DT2 led to significant decrease in TC, TG, and LDL-C and increase in HDL-C after 20,40 and 60 days in comparison with DNTC in the same period.
The results showed that the dose 2.84 g/Kg B.W caused significant decrease TC,TG,LDL-C, and an increase in HDL-C more than the dose 1.42 g/ Kg B.W. in 20 ,40 and 60 days of treatment with SAS in DT1and DT2 and there was significant decrease in TC,TG,LDL-C and VLDL-C and an increase in HDL-C in TC1 and TC2 after 60 days of treatment with SAS in comparison with NDNTC in same period .
The results showed that the dose 2.84 g/ Kg B.W. caused significant decrease in values of TC,TG,LDL-C and an increase in HDL-C than the dose 1.42 g/ Kg B.W in normal and diabetic groups.
In conclusion the result indicate the hypoglycemic and hypolipidemic effect of SAS in normal and diabetic rats.

* Effect of Gibberellic Acid and Urea Fertilizer on Some Qualitative and Quantitative Characteristics for Two Celery Cultivars (Apium graveolens L.)

Alasadi; M.J.M

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 130-143

An experiment was conducted in the winter season (2013 -2014) A.C. in house garden in the province of Dhi-Qar, to find out effects of gibberellic acid concentrations, level of urea fertilizer and their intraction on some qualitative and quantitative characteristics for two celery cultivars (Apium graveolens L.)
Experiment was designed as Randomized Comlete Block Design (RCBD) in a factorial arrangement with three replicates and organized treatments for three factors, first factor included two celery cultivars (local and imported), the second factor included four concentrations of gibberellic acid (0, 50, 100 and 150) mg. L-1 and the third factor included four levels of urea fertilizer (0, 10, 20 and 30) g. Kg-1 soil. Means were compared by using Revised Least Significant Difference (RLSD) at 0.05 probability level.
Results showed that gibberellic acid concentrations and levels of urea fertilizer influenced significantly increase leaves content of carbohydrates, nitrogen, phosphorus, potassium and total protein, as well as the percentage of volitle oil in seeds of twice cultivars also increased the qualities of yield celery plant from seed; the results showed superiority of imported cultivar in the majority of the studied characteristics while local cultivar recipes in the leaves content of total chlorophyll and the percentage of oil in the seed. Gibberellic acid concentrations caused a significant increase in all characteristics were significantly increased at the highest concentration of 150 mg. L-1 for most characteristics, while level of urea fertilizer recorded 30g. Kg-1 soil increased higher significantly in the majority of the characteristics.

Study Some Immunological and Physiological aspects in Arthritis patients

Mayada F .Drwesh

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 144-154

The present study was designed to assess some immunological and physiological aspects of arthritis patients by studying some physiological and serological parameters for 40 cases (aged 50-70 years) from AL- Sadr Teaching Hospital in AL- Najaf City Center of arthritis and physical therapy. 40 blood samble from arthritis patients which deveded into two groups : The First group consisted of (20) patients who were have high disease severity by examined the interaction with C-Reactive Protein +ve with (20) patients who were have low disease severity by examined the interaction with C-Reactive Protein –ve. Results of all required tests were obtained from the two groups compared with that results obtained from control group which included (20) healthy volunteers. Heamatological parameters included Total count of leucocytes , Hemoglobin estimation , Packed cell volume measurement , Erythrocyte sedimentation rate measurement ,Rhumatoid factor and C-Reactive Protein in addition to Specific activity of Adenosine deaminase and assess level of IL-1 concentration .
Statistical analysis of results revealed that there was a significant (P <0.05) increase in the total count of leukocytes, also found that there are a significant (P <0.05) increase in the Erythrocyte Sedimentation Rate in two groups when compared with the control group. Otherwise, the study exhibited significant decreased (P <0.05) in Hemoglobin concentration ,percentage of packed cell volume, Mean Corpuscle Hemoglobin in the two groups when compared with the control group.
Study of immunological parameters in blood serum demonstrated that there was significant increase (P <0.05) in the specific activity of adenosine deaminase as well as level of IL-1 concentration in the two groups when compared with the control group

Effect of protein deficiency on body weight, testes tissues and some parameters in male Balb/c mice

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 155-173

The present study is designed to know the effect of protein deficiency on mice males through study the changes in body and testes weight, clinical histological changes of testes. In addition of study the blood and biochemical parameters. Using 90 males of mice aging 28-30 days. These animals were divided into three groups, each group contains 30 mice, first group (control) was give 18% total protein contains animal protein 7%, while the second group was given 13% total protein contain 3.5% animal protein, and the third group was given 10 % total protein without any animal protein for different periods as follow (15,30,45) days. Results showed significant decrease (p<0.05) in weight body rate of animals in first treatment for periods (30, 45) days and second treatment for periods (15,30,45) days compared with control group , also in the rate of testes weight means in first and second treatment for periods (30,45)days compared with control group. Histological procedures showed that clinical and histological changes in both treatments for periods (30,45)days, while there is no any changes in both first and second treatment in period of ( 15) days.
In contrast, the blood parameters showed that the concentration of Hb,PCV significant decrease (p<0.05) in blood of treatment animals which correlated with protein deficiency compared with control group, and the second treatment for (45) days showed the most significant in the parameters compared with the first treatment and for all periods.
Biochemical tests obtain a significant decrease (P <0.05) in the total serum protein in the second treatment period (15, 30, 45) days compared with the control group, and found a significant decrease (P <0.05) in the first treatment in the two periods (30, 45) days compared with the control group, while did not score a significant decrease in the first treatment in the first period (15) days, compared with the control group

The Effect of Some Environmental Conditions on Phytoplanktonic Structure in AL-Diwanyia River

ad M.Alkam

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 174-191

تناولت الدراسة الحالية بعض الخواص الفيزيائية والكيميائية لمياه نهر الديوانية لمعرفة تأثير الظروف البيئية على تركيبة الهائمات النباتية في مياه نهر الديوانية اذ جمعت عينات مياه النهر من ثلاث محطات ابتداءا من تشرين الاول 2012 ولغاية ايلول 2013 اذ كانت المحطة الاولى تقع الى الشمال الغربي من محافظة الديوانية في منطقة صدر الدغارة والمحطة الثانية في مركز المدينة وتبعد عن المحطة الاولى (35)كم والمحطة الثالثة تقع جنوب المحافظة وتبعد (13) كم عن المحطة الثانية ، وتم قياس ]درجة حرارة الهواء والماء، الاس الهيدروجيني ، ، ،التوصيلية الكهربائية ،والعكورة، الاوكسجين المذاب ،المتطلب الحيوي للأوكسجين ، ،القاعدية ،العسرة الكلية ،ايونات الكالسيوم والمغنسيوم ،المغذيات النباتية (النترات ،النتريت ،الفوسفات)[ واظهرت نتائج الدراسة ان درجة حرارة الهواء والماء والاس الهيدروجيني وقيم التوصيلية الكهربائية والعكورة قد تراوحت ما بين) 15-43)م (13-33) م (7.31-(7.82 ( 1555-1264) مايكرو سيمنز /سم (NTU (59.02-43.6 على التوالي، اما قيم الاوكسجين المذاب والمتطلب الحيوي للأوكسجين فقد تراوحت ما بين ( (8.26-6.30ملغم/لتر ، (3.08-1.19)ملغم/لتر على التوالي. آما قيم القاعدية الكلية والعسرة الكلية والكالسيوم والمغنسيوم فقد تراوحت ما بين (227.4-131.5)ملغم/لتر ،((458.5-312.3ملغم/لتر ،((142-86.4ملغم/لتر ،( (76.8-54.8ملغم /لتر، اما فيما يتعلق بالمغذيات النباتية لوحظ ان النترات كانت هي الشكل السائد اذ تراوحت تراكيزها ما بين ((558.4-302.6مايكرو غرام/لتر, اما النتريت فقد تراوح ما بين((3.4-1.59مايكرو غرام/لتر ،وا لفوسفات فقد تراوحت ما بين (ND – (2.34مايكرو غرام/لتر ،كما اظهرت نتائج الدراسة الحالية ان هناك سيادة تامة وواضحة للدايتومات على باقي المجاميع الطحلبية في كل المواقع المدروسة طيلة مدة الدراسة وسجل اعلى عدد من الانواع في الموقع الاول .

Measurement copper and zinc in Al Shamia City soil Selected by using jacson method

Amer Yassir Kadhim

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 192-201

In this paper , the limited concentration copper (Cu) and zinc (Zn) were measured in Soils Selected from main and brunch streets of Shamia city in Qadisia province . The measurements were done by Atomic Absorption Spectrometer using jacson method. We find the maximum value of concentration copper was (123.48)ppm in pattern no. (3) and minimum (17.77)ppm in pattern no. (8) with average (52.18)ppm and the zinc the maximum value was (127.42)ppm in pattern no. (8) and the minimum was (27.17)ppm in pattern (4) with average (78.89)ppm The results are shown in tables and figures ..

The Immediate Effect of Crude Miswack Extract against Some Bacterial isolates from Gingivitis

Hayder Ali Muhammid

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 1-7

This study included the isolation and identification of some bacterial species which are considered as members of gingivitis in the student of Veterinary Medicine College in the University of Kerbala, Iraq . These include the of Streptococcus spp. was more involved in the third stage 70% percentage and Staphylococcus spp. was more isolated in the second stage 40% percentage by using Blood Agar medium and Manitol Salt agar, respectively. Identification of these bacteria was performed depending on morphological and biochemical crieteria, the study includes also the effect of the aqueous extract of Salvadora persica in the concentrations (20%, 15% and 10%) on the isolated bacteria which showed antimicrobial effects on these species under the study.
Biology Classification: QR75-99.5

The predication of AgNOR value in reflection of environmental pollution carcinogenesis

Thekra A. Al-Kashwan

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 8-22

The value of Nuclear organization region(AgNOR) count and polymorphism in association with carcinogens has not been clearly explained yet. The aim of study was to assess the value of mAgNOR, pAgNOR and pleomorphic analysis in bladder cancer for prediction of possibility of war pollution carcinogens in the causation of increase rates of cancer in Iraq.
A retrospective study was carried out on 100 TCC Iraqi patients classified into groups; A: include 50 cases diagnosed at 2005-2006 and B: 50 cases diagnosed at 2012-2013, and 30 cystitis as control group. After sliver staining paraffin's sections, mAgNOR and pAgNOR, and both pleomorphic and single dots were counted and analyzed.
The result shown that total mean of mAgNOR and pAgNOR for A, B and C groups were 2.6, 3.4, 1.68 and 12.4, 22.5, 1.6 respectively, with significantly increase in B group in comparisons with others (p≤0.05). mAgNOR was significantly higher in low grades and stage of B group in comparison with A group (p≤0.05), while pAgNOR was observed more frequently in both low and high grade and stage in this group rather than the other patient group (p≤0.05). overall mean of AgNOR was not significantly increased in B group in comparison with others, but AgNOR pleomorphism was significantly larger in low grade and stage in B group than in another malignant group(A) (p≤0.05)., and significantly associated with high grade and stage of this group only (p≤0.05).
mAgNOR and pAgNOR counts with along AgNOR pleomorphism can be useful markers of cellular kinetic to predict the effect of war pollution carcinogens. pAgNOR and AgNOR pleomorphism cellular proliferation markers have more predicative value in characterizing cell kinetic. Further large study will be needed to confirm these results.

Preliminary Phytochemical and Screening of Biocomponents by GC-MS Technique in Myrtus communis L. Plant Flowers

Dhafer A. K. Jamil

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 23-33

Phytochemical components of Myrtus communis L. flowers have been evaluated using GC-MS. The chemical compositions of M. communis L. flowers were investigated using Shimadzu Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched by the National Institute of Standards and Technology (NIST) library. The qualitative analysis of ethanolic extract of M. communis L. flowers showed that alkaloid, tannin, phenol, flavonoids, terpenoids, sapanoin, anthraquinones, protein and quinones present in ethanolic extract. GC-MS analysis of ethanolic extract of M. communis L. flowers revealed the existence of acetic acid, camphor, Bicyclo[2.2.1]heptane, 2-methoxy-1,7,7-trimethyl-, 2-Butenoic acid, 2-methyl-, (Z)-, 1-Hexadecanol, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, 1,3-Cyclopentadiene, 5,5-dimethyl-2-ethyl-, Diazoacetic acid, 2-isopropyl-5-methylcyclohexyl ester, 3,6-Octadien-1-ol, 3,7-dimethyl-, (Z)-, 3-Oxabicyclo[4.1.0]heptan-2-one, 4,4,7,7-tetramethyl-, 12-Oxabicyclo[9.1.0]dodeca-3,7-diene, 1,5,5,8-tetramethyl-, 3-Buten-2-one, 4-(2-hydroxy-2,6,6-trimethylcyclohexyl)- and thujone. The quantitative determination of an ethanolic extract of M. communis L. flowers contains high concentrations of phenol (7.84 ± 0.69 mg/gm), flavonoids (5.23 ± 0.56 mg/gm), tannin (2.04 ± 0.43 mg/gm), and saponins (1.83 ± 0.49 mg/gm). The results of this study offer a platform of using M. communis L. an herbal alternative for various diseases.

Resistance of Pseudomonas aeruginosa to multiple β-lactam antibiotics

Venus Hassan Abdul Amir

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 34-40

A total of 90 Pseudomonas aeruginosa (P.aeruginosa) strains clinical samples were collected from burn, ear, and foot diabetic samples in Al-Hilla, and Marjan teaching hospitals were identified to species level with a VITEK-2 system. The susceptibility to 11 antibiotics were tested using disc diffusion test resulting in 55.4% of 90 P.aeruginosa isolates were resistant to 11 antibiotics. Cefinase discs impregnated with nitrocefin method was used for the detection of β-lactamase production in antibiotics resistant 90 P.aeruginosa isolates. Results showed that 56% isolates were able to produce β-lactamase.

Antimicrobial activity of chitosan isolated from fungus Rhizopus oryzae

Kawthar Tuma Khalaf

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 41-48

In this study chitosan was isolated from the fungus Rhizopusorayzae by using yeast peptone glucose broth medium (YPG) at the late stationary phase. Chitosan production from mycelium reached to 15 mg/g as dry weight . 25% was the N-acetylation of chitosan while 96% for the D-glucosamine. Chitosan showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Candida albicans and reach to 20 , 15 and n 15 mm as inhibition zone respectively

Comparison of a Seven Conventional Phenotypic Methods with Polymerase Chain Reaction for Detection of Methicillin-Resistant Staphylococcus aureus

Ali M. Al-Mohana

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 49-63

Early detection of methicillin-resistant Staphylococcus aureus (MRSA) is critical for both the management of infected patients, and the timely institution of appropriate infection control measures. Although detection of the mecA gene by polymerase chain reaction (PCR) remains the gold standard, this technology is inaccessible for many laboratories. Therefore, this study sought to evaluate seven phenotypic methods and compare them with PCR. A total of 135 S. aureus were collected from 343 clinical samples between August 2012 and January 2013 from several clinical sources that were randomly selected from patients in three main hospitals in Al-Najaf city. MRSA isolates were identified using PCR with primers specific for the mecA gene. PCR was used as the reference method, thus, the prevalence of MRSA in Najaf hospitals was 64 (47.4%) and the remaining 71 (52.6%) isolates were mecA-negative methicillin sensitive S. aureus (MSSA), and all MRSA isolates were tested for comparison using cefoxitin and oxacillin disc diffusion, oxacillin and methicillin HiComb E-test, oxacillin screen agar, BBLTM CHROMagarTM MRSA, HiCromeMeReSa agar. The findings of this study revealed that FOXDD had a high accuracy (97.8%) comparative to other phenotypic methods tested for detection of MRSA followed by OX (E-test), CHROMagar, HiCrome agar (97%), OXDD (96.3%). The OXSA (93.3%) and MET E-test (88.9%) had the lowest concordance with PCR results. Finally, FOXDD method may be preferred in this study and in other clinical laboratories because it is highly accuracy, easy to perform, low cost, and does not require special equipment.

Naproxen Sodium Release from Poly(acrylic acid-co- N-vinyl-2- pyrrolidone) Hydrogels

Nadher D. Radhy

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 64-76

Copolymer hydrogels of acrylic acid (AA) and N-vinyl-2-pyrrolidone (NVP), crosslinked
with 1,4-butanediol dimethacrylate (BDDMA), were prepared by radical copolymerization.
The chemical structures of copolymer hydrogels were analyzed by Fourier transform
infrared spectrometer (FTIR) and H NMR spectroscopy. The swelling ratio (Rs) was
measured for four types of hydrogels structures, in three different media pH 1.2 , 4 and 7.4
at 370C as a function of time. A Naproxen Sodium is used as a model drug to investigate
the drug release profile of the hydrogels. The concentration of drug released was measured
on UV-Vis spectrophotometer. Results indicated that the swelling ratio and drug release is
increase concentration decrease of crosslinked agent and pH increase.

Temperature Effect on the Effective Secondary Emission Coefficient for He and Ne Gases

Salman Hamza Hussein

journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 77-89

The relation between the effective secondary emission coefficient (ESEC)  and
temperature has been studied for He and Ne atoms. So we are studied the variation of 
withreduced electric field to the gas pressureE p between electrodes at different temperature (
T ) for each gas.It has beenfound that the ESEC increase with ( E p ) at constant (T ) and
decreases with temperature at constant ( E ).

Calculating the Temperature dependence Radiative recombination rate Coefficients for ions


journal of al-qadisiyah for pure science(quarterly), Volume 16, Issue 1, Pages 90-96

Radiative recombination rate coefficients (RRC) are presented for several ions with open and closed
shells, ( H I, He I, He II, N V, N VI, N VII) . The temperature of electron range 10-109 K is considered.
The calculations have been performed in the framework of analytic fit parameters formula with no
consideration of relativistic effects. Our results of RRC compared with the theoretical data available as a
function of electron temperature, and the agreement was very good. Some data which had been depended
showed a peak at high temperatures because of the influence of autoionizing resonance.