journal of al-qadisiyah for pure science(quarterly)

Abstract

This study included the efficacy of the alkaloid , which extracted from leaves of Conocarpus
erectus and fruits , seeds of Citrullus colocynthis and urea on the radial growth of Alternaria
alternata and Aspergillus ochraceus isolated from seeds and roots of faba bean compared
with fungicide Tapsin, also testing the effect of these treatment in germination of seeds in
sterilized and un sterilized soil , and detection of tested fungi by molecular method using
polymerase chain reaction( PCR). The results showed that the alkaloids have significant effect
on growth of tested fungus on solid culture medium (PDA) , in measuring with controlled
treatment and tested fungicide at level of possibility 5%, and also increasing the rate of
germination of faba bean seeds in sterilized and un sterilized soil. The concentration 15mgml
had the greatest action comparing with other alkaloids and urea concentrations and
fungicides. The testing of measuring Infra-Red Spectrum (FTIR) showed the presence of
many specific band due the extraction alkaloids.