journal of al-qadisiyah for pure science(quarterly)

Abstract

The study was conducted in Animal house of the department of Biology, College of
Education, Al-Qadisiya University during the period from 1/9/2013 to 1/9/2014. The study
diagnosed the parasite of E.tenella in naturally infected chickens in poultry farms. E.tenella was
isolated from the infected cases in Vet. hospital and Vet. clinics.Then,the cases diagnosed initially
in the laboratory direct examining stool by ordinary microscope and the positive cases were
recorded and diagnosed by using the technique of Conventional Polymerase Chain
Reaction(PCR). There are 315 cases of infected chicken which are examined and the results
showed that (186 out of 315) stool Samples,59%, The experimental infection for each experiment
was done in age 21 day with dosage of (5×104) grown oocysts/chicken for E.tenella in two types
of chickens(Ross and local breed) Also,two groups of uninfected chickens (control) are used for
the sake of comparison.
Hematological parameters in Ross chickens showed significant decrease (p<0.05) in fourth
week of infection), percentage of Packed cell volume (PCV)(18.47% in the fifth week of
infection) and Red blood cell (RBC) count was (1.58x106 in the fourth week of infection) . Also ,
the infection led to significant difference of white blood cell (WBC) count (30.19x103 in the
seventh week of infection) comparison of uninfected chickens of the control group. While The
hematological parameters in local breed showed significant decrease (p<0.05) in Hb
concentration and percentage of PCV (7.13mg/100ml , 21.26% respectively in the fifth week of
infection) , while the RBC count was (2.05 x 106 in the fourth week of infection and significant
increased (p<0.05) in WBC count was (30.63 x 106 the sixth week of infection) in comparison
with the chickens of the control group.