Keywords : urinary tract infection

enotypical and phenotypical detection of hemolysin enzyme of Escherichia coli causing urinary tract infections

Azhar N. Hussein

journal of al-qadisiyah for pure science(quarterly), 2017, Volume 17, Issue 1, Pages 118-128

The present study included isolation and diagnosis of the bacteria Escherichia coli from
patients suffering urinary tract infection symptoms in different ages and both sexes, which was
taken from the consulting patients in Maternal Hospital, and General Teaching Hospital in ALDiwianyia city during the period from 1/3/2012 till 1/8/2012 .
The results of morphological and biochemical tests appeared that 94 isolates belong to bacteria
E. coli , among them 40 (42.6%) isolates were hemolysis on blood agar plates containing human
blood ( A, B, AB, O ) and were production percentage of hemolysin (55%, 72.5%, 20%, and 40%)
respectively .
Some virulence factors, which bacteria have, were studied in both phenotypical and genotypical .
Such as hemolysin production . The results showed that all the bacterial samples 40 (100%) were
hemolysin production phenotypically as well as had the chromosomal genes hlyA and hlyB 40
(100%) and also had the plasmid gene hlyA plasmid 36 (90%) by using the polymerase chain
reaction for detection about these genes .
The results of electrophoresis on Agarose gel showed that most of the bacterial isolates contain
plasmid bands between one to three 36 (90%) . All isolates contained one plasmid band large in
size, it molecular weight was about 1200 bp 36 (90%) isolate, the plasmid bands distributed among
one plasmid band 14 (35%) isolates, two plasmid band 19 (47.5%) isolates, and three plsmid band 3
(7.5%) isolates .
The results of bacterial conjugation showed the possibility of transmitting of plasmids among
E.coli giving isolates and E.coli MM294 receiving strain in complete process . The features of the
conjugation cells were studied as having virulence factor phenotypically included hemolysin
production, and also were detected about hlyA plasmid the results showed that isolates were
contained of that gene .

Incidence of urinary tract infection (UTI) among pregnant women in Al-Muthanna Province

Taisir Abdulelah kadhim

journal of al-qadisiyah for pure science(quarterly), 2013, Volume 13, Issue 4, Pages 25-35

This study reported the incidence of urinary tract infections (UTIs) among 64 pregnant women (50 patients and 14 controls) conducted in AL-Samawa Teaching Hospital of Gynecology and Pediatrics, as well as the isolation and identification of the pathogens responsible for the infection. Significant bacteriuria is defined as greater than 1 ͯ 105 of the same bacteria per milliliter of urine. A total of 64 clean voided mid-stream urine samples were collected from pregnant women between the ages of 13-40 years. The results showed that the incidence of UTIs in this study population was 66%, and 33 bacterial isolates (20 single cultures and 13 mixed cultures) were identified based on colonial size, color characteristics, according to HiCrome UTI agar. The most predominant bacterium was Staphylococcus aureus (ATCC 25923) 12 (36.4%). This was followed by Enterococcus faecalis (ATCC29212) 4 (12%), Pseudomonas aeruginosa (ATCC 27853) 2(6), Klebsiella pneumonia (ATCC 13883) 1 (3%), Escherichia coli (ATCC 25922) 1 (3%), and a mixed culture of Enterococcus faecalis (ATCC 29212) and Staphylococcus aureus (ATCC 25923) 8(24.2%), Escherichia coli (ATCC 25922) and Enterococcus faecalis (ATCC 29212) 2(6%), Staphylococcus aureus (ATCC 25923) and Klebsiella pneumonia (ATCC 13883) 2(6%) , Enterococcus faecalis (ATCC 29212) and Klebsiella pneumonia (ATCC 13883) 1(3%) . All bacterial species exhibited 100% susceptibility to Amikacin and Imipenem whereas showed 100% resistance to Ampicillin, Erythromycin, Cephalothin, Cephalexin, Augmentin, Kanamycin, Tobramycin, and Neomycin .

Prevalence of rubella among aborted and pregnant women in Wasit Province in relation to congenital anomalies in embryos and children

Rasha Noori Al-shammary; Samah Ahmed Kadum; Rasha Hadi Saleh; Nabeel Sahib Majeed; Abdul Amir Samir Saadoun; Taisir Abdulelah kadhim; Ali M. Saad Al-khafaji; Ghazi M. Al-Maliki; A. A; Yaseen; Samer M. Al-Hulu; Samah Ahmed Kadum; Azhar N. H. AL Mussawy; Mohammed Z. Jassim; Saad M. Potrous; Musawi; Hussein M .Ali Al; Heiyam Najy Hady; Salim dawood Mohisn; NOOR ALI HUSSEIN; Dunya Mohamed Hammed; Ahmed Sallal Joudah; Wisam A.Radhi; Nadia Sadik Majeed; Layla. Ali. Mohammad; Azhar A. Ghali

journal of al-qadisiyah for pure science(quarterly), 2013, Volume 13, Issue 4, Pages 1-10

Serological examination to detect Rubella virus and ultrasound examination to known congenital anomalies in embryos among aborted and pregnant women blood sample of 367 and children blood sample of 41 was carried out in Al – Karama hospital and AL-Zahra hospital of Wasit province from June to September 2011 and January 2012. Overall prevalence of Rubella (positive cases) was 220 (53.9%). Prevalence of virus increased in age (23-29) and there were highly significant (P